Document Type : Research Note
Authors
1
Arak University, Department of Biology, Faculty of Sciences
2
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
3
Department of Biology, Faculty of Sciences, Arak University, Arak 38156-8-349
Abstract
Salsola arbuscula as a halophytic plant which is tolerant to high salinity stress. The present study is an attempt to determine the extent to which the callus of this plant is tolerant to salt stress indicated by the amounts of proline, total protein, carbohydrates and inorganic ions including Na+, K+, Cl- and Ca2+. The plant seeds were cultured in MS medium for 2 months and the resulted seedling explants were transferred to media with different hormonal concentrations. Then the produced calli were transferred to the previous medium with 0, 100, 200, 300, 400 mM of NaCl. Being kept in the culture room at 25 ± 2ºC with a 12-h photoperiod (irradiance of 800 µmolm-2S-1) for one month, calli were measured in terms of their salt stress parameters. The obtained results indicated that the best medium for callus induction was MS + 2,4-D (1mg/L) + Kin (1mg/L). The proline, protein, carbohydrate, sodium and chloride levels of concentration increased up to 300 mM of NaCl, but decreased significantly in the 400 mM of NaCl. However, potassium and calcium ion concentrations and K+/Na+ ratio reduced noticeably in the salt-stressed calli compared to control medium. It should be noted however that in terms of K+/Na+ ratio, the various salt treatments did not differ significantly from each other, thereby, indicating callus tolerance to salt stress. Thus, salt stress tolerant callus is not because of vacuolar compartmentation, but rather is due to increasing osmolarity and synthesis of different transcription factors and stress proteins.
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