Non-clonal dissemination of Extended-spectrum beta lactamase-producing Pseudomonas aeruginosa strains of clinical origin

Articles in Press

Document Type : Regular Paper

Authors

1 Department of Biosciences, Integral University, Lucknow-226026, India

2 KACST Technology Innovation Center for Personalized Medicine at King Abdulaziz University, Jeddah, Saudi Arabia

3 Department of Bio-engineering, Integral University, Lucknow

Abstract

Monitoring the emergence and transmission of Pseudomonas aeruginosa strains is important for infection control worldwide. The aim of this study was to determine the genetic diversity of 47 extended spectrum beta-lactamase producing P. aeruginosa strains of clinical origin by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and also to ascertain the presence of class 1 integrons amongst them. ERIC sequences are 127-bp imperfect palindromes that occur in multiple copies in the genomes of enteric bacteria. The ERIC-PCR profile generated showed polymorphism in the studied strains. Based on the ERIC-PCR profile, P. aeruginosa strains were grouped into 23 types. The major genotype designated as type L1, accounted for 10.6% (5/47) of samples. Class 1 integrons were detected in 8 of the 47 clinical isolates. Further, the integron-carrying isolates were found to display higher minimum inhibitory concentration against tested antibiotics. Optimization of antimicrobial use and control of infection is recommended to prevent the increase in the population of drug resistant organisms.

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Iranian Journal of Science

Articles in Press, Accepted Manuscript
Available Online from 04 January 2016

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