Isolation and identification of cellulolytic and ethanologenic bacteria from soil

Document Type : Regular Paper


1 Microbial Biotechnology Laboratory, Department of Zoology, University of the Punjab, Lahore Pakistan

2 Department of Zoology, University of the Punjab, Lahore Pakistan.


In this study, five different strains of bacteria were isolated and identified from soil samples cultivated on sugarcane bagasse as substrate in submerged fermentation. These bacterial isolates were identified by 16S rRNA gene sequencing which revealed that they were Bacillus pumilis, Bacillus lichniformis, Paenibacillus dendritiformis, Bacillus cereus and Pseudomonas aeruginosa. These bacteria were screened for cellulolytic and ethanologenic potentials using sugarcane bagasse as substrate. All the bacteria were found cellulolytic and ethanologenic. Bacillus pumilis strain produced maximum cellulase while Bacillus cereus yielded maximum ethanol. The results indicated that these strains could be used for second generation biofuels production.


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