Effect of KOH pretreatment on lignocellulosic waste to be used as substrate for ethanol production

Document Type : Regular Paper


1 Food & Biotechnology Research Center (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex Ferozpure road Lahore Pakistan

2 University of the Punjab


In present study, Leptochola fusca (kallar grass) and Gossopium herbarium (cotton stalk) were treated with various concentrations of KOH (1%, 1.5%, 2% 2.5% and 3%) for different soaking periods (6h, 12h, 24h and 48h) to expose maximum cellulosic content. The results showed that maximum cellulose exposure and delignification was increased with the increase in concentration of alkali and soaking time. However, maximum cellulose content (Kallar grass 49.01±0.1%, Cotton stalk 56.12±0.1% ) was achieved with 2.5% KOH at 24h and a decline was observed as further increased in concentration of KOH (3%). Moreover maximum delignification (Kallar grass 66.2%, 62.6 cotton stalk) was observed at 3% KOH after 48 h of soaking period. However, these results showed adverse effect of higher concentration of alkali on holocellulose. The cellulosic content of Kallar grass and cotton stalk after pretreatment at suitable conditions indicated that these substrates could be used as promising substrates for saccharification process to produced second generation biofuel.


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