In vitro antioxidant and antimicrobial activity of some Lamiaceae species

Document Type : Regular Paper


1 Department of Biology, Science Faculty, Erciyes University, 38039 Kayseri,Turkey

2 Ulubey Vocational College, Ordu University, 52850 Ordu, Turkey

3 Department of Food Engineering, Chemical and Metallurgical Engineering Faculty, Yıldız Teknik University, 34210 İstanbul, Turkey


The methanolic extracts, infusions, decoctions and hydrosols of six plants were investigated for their total
phenolic contents, antioxidant and antimicrobial activities:
Mentha piperita (Peppermint),
Thymus vulgaris
Melissa officinalis (lemon balm), Ocimum basilicum (basil), Rosmarinus officinalis
(rosemary) and
Salvia officinalis
(sage) (Lamiaceae). Total phenolic contents were determined by Folin-Ciocalteu procedure and
ranged from 111.03 ± 0.6 (sage methanolic extract) to 19.07 ± 0.0 mg (basil methanolic extract) gallic acid
equivalent /g extract. The antioxidant activity was evaluated by two methods, DPPH and phosphomolybdenum
assays. The decoction of rosemary showed the highest DPPH radical scavenging activity (IC
= 8.36 μg/mL). The
methanolic extract of Peppermint showed the highest total antioxidant activity (241.85 ± 1.9 mg ascorbic acid
equivalent /g extract) measured by phosphomolybdenum assay. The antimicrobial activities of herbs were tested
against eight bacteria and two yeasts.