Optimization of the key medium components and culture conditions for efficient cultivation of Geobacter sulfurreducens PCA ATCC 51573 using response surface methodology

Document Type: Regular Paper

Authors

1 Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia , 43600 UKM Bangi, Selangor, Malaysia.

2 Department of Microbiology, Masjed Soleiman Branch, Islamic Azad University, Masjed Soleiman, Iran

3 Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (UKM) , 43600 UKM Bangi, Selangor, Malaysia.

4 Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (UKM) , 43600 UKM Bangi, Selangor, Malaysia

5 School of Biosciences and Biotechnology, Faculty of Science and Technology, National University of Malaysia (UKM) , 43600 UKM Bangi, Selangor, Malaysia.

Abstract

Microbial electrolysis cell (MEC) is a promising method for hydrogen production by microorganisms. The culture conditions were optimized for Geobacter sulfurreducens PCA ATCC 51573 using response surface methodology involving central composite design (CCD). Effects of sodium bicarbonate, sodium acetate, sodium fumarate, incubation temperature and initial pH value of culture on cell growth of G.sulfurreducens PCA ATCC 51573 were studied. Experimental data obtained from CCD was utilized to generate a second-order polynomial regression model and to evaluate the effect of the variables studied on the optical density (OD) as the main response of bacterial growth of the strain PCA ATCC 51573. The statistical analysis showed sodium bicarbonate, sodium acetate and sodium fumarate had significant effect on OD value (P

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