• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Editorial Staff
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter Telegram
Iranian Journal of Science and Technology (Sciences)
Articles in Press
Current Issue
Journal Archive
Volume Volume 39 (2015)
Issue Issue 4
Issue Issue 3.1
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 38 (2014)
Volume Volume 37 (2013)
Volume Volume 36 (2012)
Volume Volume 35 (2011)
Volume Volume 34 (2010)
Volume Volume 33 (2009)
Volume Volume 32 (2008)
Volume Volume 31 (2007)
Volume Volume 30 (2006)
Volume Volume 29 (2005)
Volume Volume 28 (2004)
Golijani Moghadam, R., Yazdanpanah-Samani, M., Zamani, M., Motallebi, M. (2015). Heterologous expression of chit36 gene from Trichoderma atroviride in canola reduces development of lesion caused by Sclerotinia sclerotiorum. Iranian Journal of Science and Technology (Sciences), 39(3), 331-339. doi: 10.22099/ijsts.2015.3158
R. Golijani Moghadam; M. Yazdanpanah-Samani; M. R. Zamani; M. Motallebi. "Heterologous expression of chit36 gene from Trichoderma atroviride in canola reduces development of lesion caused by Sclerotinia sclerotiorum". Iranian Journal of Science and Technology (Sciences), 39, 3, 2015, 331-339. doi: 10.22099/ijsts.2015.3158
Golijani Moghadam, R., Yazdanpanah-Samani, M., Zamani, M., Motallebi, M. (2015). 'Heterologous expression of chit36 gene from Trichoderma atroviride in canola reduces development of lesion caused by Sclerotinia sclerotiorum', Iranian Journal of Science and Technology (Sciences), 39(3), pp. 331-339. doi: 10.22099/ijsts.2015.3158
Golijani Moghadam, R., Yazdanpanah-Samani, M., Zamani, M., Motallebi, M. Heterologous expression of chit36 gene from Trichoderma atroviride in canola reduces development of lesion caused by Sclerotinia sclerotiorum. Iranian Journal of Science and Technology (Sciences), 2015; 39(3): 331-339. doi: 10.22099/ijsts.2015.3158

Heterologous expression of chit36 gene from Trichoderma atroviride in canola reduces development of lesion caused by Sclerotinia sclerotiorum

Article 12, Volume 39, Issue 3, Summer 2015, Page 331-339  XML PDF (3247 K)
Document Type: Regular Paper
DOI: 10.22099/ijsts.2015.3158
Authors
R. Golijani Moghadam; M. Yazdanpanah-Samani; M. R. Zamani* ; M. Motallebi
National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, Iran
Abstract
 
An endochitinase gene (chit36) was isolated from the biocontrol fungus Trichoderma atroviride. Chit36 was overexpressed under the CaMV35S constitutive promoter in canola. Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens. The insertion of transgene was verified by PCR and DNA dot blotting. RT-PCR analysis indicated that the transgenic canola was able to express chit36 gene. Also, we used a transgenic over-expression approach in order to investigate antifungal activity of expressed chit36 on Sclerotiana sclerotiorum. Antifungal activity was detected in crude protein extracts from transgenic canola using radial diffusion assay. Also, lesion sizes of transgenic canola by S. sclerotiorum were significantly retarded compared to non transgenic canola plant using detached leaf assay.
Keywords
Antifungal activity; canola; chit36; Sclerotiana sclerotiorum
Statistics
Article View: 1,411
PDF Download: 1,987
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by sinaweb.