DETERMINATION OF PLOIDY LEVELS OF SOME POPULATIONS OF AGROPYRON CRISTATUM (POACEAE) IN IRAN BY FLOW CYTOMETRY

Document Type: Regular Paper

Authors

1 Department of Biology, Universities of Isfahan and Payam Noor, Isfahan, I. R of Iran

2 Department of Biology, University of Isfahan, Isfahan, I. R of Iran

Abstract

Flow cytometry (FCM) was used to determine the ploidy levels of six different populations
of Agropyron cristatum (L.) Gaertn. (Poaceae) in Iran, using a diploid cultivated barley (Hordeum
vulgare L.) as an internal reference. According to flow cytometric data, tetraploid (2n = ± 28) and
hexaploid (2n = ± 42) levels were detected among the examined populations and these results were
supported by chromosome counting on the same materials included in this study. The mean nuclear
DNA content (2C value) of the populations were then estimated, ranging from 26.41 to 27.56 pg for two
varieties of Agropyron cristatum subsp. pectinatum, and 43.47 pg for Agropyron cristatum subsp.
incanum. The relationships between morphological variations observed among the taxa and ploidy levels
were also discussed.

Keywords