EVALUATION OF THE CHLROPLAST DNA AMONG VICIA FABA L. GERMPLASM USING RESTRICTION- SITE ANALYSIS

Document Type: Regular Paper

Authors

1 1Department of Crop Science, Faculty of Agriculture, ShahreKord University, ShahreKord, I. R. of Iran, 8818634141

2 Department of Biology, Falavarjan Branch, Islamic Azad University

Abstract

A restriction-site analysis of chloroplast DNA (cpDNA) was carried out to evaluate the level
of diversity in Vicia faba L. germplasm selected from different geographical regions using 11 restriction
endonucleases. We analyzed 214 restriction sites in 18 accessions of Vicia faba. All of the accessions
had identical cpDNAs, pointing out the ancestral character of all the accessions are of only one gene
pool, and all of them must have evolved through the same maternal lineage. Molecular size of the
chloroplast DNAs obtained was 123.25 kb, indicating that it had lost one of the inverted repeats. Due to
lack of cpDNA diversity, it is concluded that the broad bean has passed through a genetic bottleneck
during domestication and lost most of its cytoplasmic variability. The present study favors the
monophyletic origin of its cytoplasm, and accordingly, of Vicia faba.

Keywords