ISOENZYME CHARACTERIZATION OF IRANIAN LEISHMANIA ISOLATES FROM CUTANEOUS LEISHMANIASIS

Document Type: Regular Paper

Authors

1 Department of Parasitology, Shiraz University of Medical Sciences, Shiraz, I. R. of Iran

2 Faculty of Medicine, Department of Parasitology and Mycology, 19, Street Tarik Ibn Ziad, Casablanca, Morocco, 20006

3 Department of Parasitology, Isfahan University of Medical Sciences, Isfahan, I. R. of Iran

Abstract

During the period from 1996 to 1997, 45 isolates of Leishmania were recovered from
patients suspected of cutaneous leishmaniasis (CL) and one from a gerbil. These specimens were
received from various parts of Iran. Isoenzyme profiles of these isolates were compared with those of
reference strains of L.tropica, L.major and L.infantum using cellulose acetate electrophoresis (CAE) and
10 enzyme systems (MDH,ME, NH1, NH2, PGM, GPI, 6PGD, GOT, SOD and G6PD). The isolate
obtained from the gerbil was characterized as L.major. From the patients with CL, 27 isolates were
characterized as L.tropica, 10 as L.major and 8 remained undetermined.

Keywords