THE EFFECTS OF UV-A, UV-B AND UV-C ON PROTEIN AND ASCORBATE CONTENT, LIPID PEROXIDATION AND BIOSYNTHESIS OF SCREENING COMPOUNDS IN BRASSICA NAPUS

Document Type: Regular Paper

Authors

Biology Department, Shahid Bahonar University of Kerman, Kerman, I. R. of Iran

Abstract

The role of the antioxidant defense system was studied in Brassica napus, and subjected to
different supplementary bands of ultraviolet radiation (UV-A, UV-B and UV-C). This study showed that
UV-B and UV-C induced oxidative stress as evidenced by an increase in lipid peroxidation and total
ascorbate content. Under UV-B and UV-C treatments, flavonoids and anthocyanins were increased
significantly compared with control and UV-A treated plants. These compounds act as internal filters
and also have an antioxidant activity against active oxygen species such as H2O2. Results of this research
showed that the protein content increased under UV-B and UV-C. These increments are probably related
to the synthesis of defense proteins such as HSP, the expression of genes that are encoding the
antioxidant enzymes, or those which contribute in UV-absorbing compound synthesis pathways such as
PAL, CHS and CHI.

Keywords