Isolation, identification, biochemical properties and potential application of an organic solvent-tolerant lipase from Pseudomonas sp. strain NEB-1

Document Type: Regular Paper

Authors

1 Molecular Biotechnology Lab., Department of Biology, Faculty of Sciences, Shiraz University, Shiraz 71454, Iran

2 Institute of Biotechnology, Shiraz University, Shiraz, Iran

Abstract

Using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from
areas contaminated by oil and organic solvents. Among the strains, 15 isolates exhibited extreme stability toward
organic solvents and high lipolytic activity. The NEB-1 isolate which was later identified as
 
Pseudomonas sp.
strain NEB-1 by biochemical tests and 16S rDNA gene sequence analysis was selected based on extremely high
tolerance to organic solvents and maximum lipase production. Biochemical studies revealed that the crude lipase
was stable at temperatures between 20 ºC and 60 ºC and pH ranges of 4 to 11 for 1 h. Optimum pH and
temperature of the enzyme were revealed to be 9.5 and 70 ºC, respectively. The crude lipase showed remarkable
tolerance in presence of different organic solvents with a broad range of hydrophobicity characteristics. The
solvent stable lipase showed an attractive potency for application in biocatalysis in non-aqueous systems and
biodiesel production.

Keywords