Heteroalkyl oxime derivative can cause damage to bacterial DNA

Document Type: Research Note

Authors

1 Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran

2 Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran

3 Department of Medicinal Chemistry, Shiraz University of Medical Sciences, Shiraz, Iran

4 Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran

Abstract

The present study aimed to evaluate the antimicrobial activity of a hetroalkyl oximec synthetic compound
derivative (Benzophenone O-4-bromoethyl oxime) against two gram positive and three gram negative bacteria.
The antibacterial characteristic of this compound was shown using the circular zone diameter of bacterial growth
inhibition by disk-diffusion method. In addition, the random amplified polymorphic DNA (RAPD) technique
using twenty-three primers was performed to investigate the DNA polymorphism of bacteria that were affected by
the synthetic chemical drug. Among these, only 18 primers were found efficient for reproducible banding pattern.
Disappearance or appearance of bands between controls and treatments confirm rearrangements and DNA
damages in the priming binding sites of bacterial genome. The results show that the RAPD method can be a useful
identification tool for studying the DNA polymorphism created by synthetic chemical drugs. The results obtained
with this technique showed significant differences between the RAPD profiles.

Keywords