Effect of ±5ºC difference from physiologic temperature on surface area, volume, and surface-to-volume ratio of transformed cells

Document Type: Regular Paper

Authors

1 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Department of Biology, Islamic Azad University, Garmsar Branch, Garmsar, Iran

3 Human Nutrition Department, Faculty of Nutrition, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Information on the relationship between culture temperature and changes in the cell's area, volume and area to volume (S/V) ratio is lacking. Cancerous epithelial-like cell lines SW480, SW742 and T-47D were cultured at 32, 37 and 42°C for 48hr. Digital images of cells were analyzed using software Imaged. Observed surface area, volume changes, and surface area to volume (S/V) ratio were significantly different at 32, 37 and 42°C in the three cell-lines tested (P<0.05). S/V ratios for all three cell lines at 37°C were significantly greater than that of at 42°C (P=0.0001).