@article { author = {Kadier, Abudukeremu and Abdeshahian, Peyman and Kalil, Mohd Sahaid and Hasan, Hassimi Abu and Hamid, Aidil Abdul}, title = {Optimization of the key medium components and culture conditions for efficient cultivation of Geobacter sulfurreducens PCA ATCC 51573 using response surface methodology}, journal = {Iranian Journal of Science}, volume = {}, number = {}, pages = {-}, year = {2016}, publisher = {Springer}, issn = {2731-8095}, eissn = {2731-8109}, doi = {10.22099/ijsts.2016.3868}, abstract = {Microbial electrolysis cell (MEC) is a promising method for hydrogen production by microorganisms. The culture conditions were optimized for Geobacter sulfurreducens PCA ATCC 51573 using response surface methodology involving central composite design (CCD). Effects of sodium bicarbonate, sodium acetate, sodium fumarate, incubation temperature and initial pH value of culture on cell growth of G.sulfurreducens PCA ATCC 51573 were studied. Experimental data obtained from CCD was utilized to generate a second-order polynomial regression model and to evaluate the effect of the variables studied on the optical density (OD) as the main response of bacterial growth of the strain PCA ATCC 51573. The statistical analysis showed sodium bicarbonate, sodium acetate and sodium fumarate had significant effect on OD value (P}, keywords = {Microbial electrolysis cell,Geobacter sulfurreducens PCA,Response surface methodology,Culture optimization,Optical density (OD)}, url = {https://ijsts.shirazu.ac.ir/article_3868.html}, eprint = {} }